For instance, positive displacement pipettes are ideal for handling volatile, viscous, and other kinds of difficult samples. In addition, you need to know whether you are using the right kind of pipette for your workflow. For instance, if you are pipetting 2µL of the sample, use a 2.5-5 µL pipette rather than a 10µL one. Be sure to choose the smallest possible pipette for handling the sample volume. To mitigate these errors, be sure to select the proper pipette that delivers volumes that match your protocol. Volumetric errors are amplified at such low volumes. Here are some tips for pipetting small volumes accurately. If you want consistent results while working with small liquid volumes, you need to identify and eliminate all potential sources of error and review your lab workflow on a regular basis. While working with small volumes, factors such as temperature and dispensing accuracy are critical to control and manipulate. In order to detect microscopic changes accurately in small sample volumes, test tubes and vials are being gradually replaced by microplates, which require reagents in the microliter/nanoliter range. Have you ever meticulously planned your workflow, only to find that your results have too much variance, forcing you to redo your experiment? As modern researchers increasingly work with high-value small volume samples, the need for advanced pipetting techniques/protocols is evident.
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